Document 0590
 DOCN  M9480590
 TI    HIV-1 gp41 enhances major histocompatibility complex class I and ICAM-1
       expression on H9 and U937 cells.
 DT    9410
 AU    Chen YH; Bock G; Vornhagen R; Steindl F; Katinger H; Dierich MP; Ludwig
       Boltzmann Institute for AIDS Research, Innsbruck, Austria.
 SO    Int Arch Allergy Immunol. 1994 Jul;104(3):227-31. Unique Identifier :
       AIDSLINE MED/94305372
 AB    Based on our findings that HIV-1 (human immunodeficiency virus type 1)
       soluble gp41 (sgp41; amino acids 539-684) bound to human T, B, and
       monocyte cells and enhanced major histocompatibility complex (MHC) class
       I and II antigen expression on Raji cells, we examined the effect of
       HIV-1 sgp41 on the surface expression of MHC I and II, ICAM-1, and CD4
       molecules on human H9 and U937 cells. Flow cytometry (FACS) analysis
       demonstrated that sgp41 selectively enhanced MHC class I expression by
       about 75% on H9 cells and by about 85% on U937 cells, while the ICAM-1
       expression was increased by about 70% only on H9 cells and remained
       unaltered on U937 cells; other molecules, such as MHC class II and CD4,
       remained unaltered. By comparison, alpha-, beta-, and omega-interferons,
       but not gamma-interferon, showed similar effects as sgp41 on the
       expression of MHC class I and ICAM-1 on H9 and U937 cells. The results
       suggest that HIV-1 gp41 may have a biological function that is involved
       in the regulation of human MHC class I and ICAM-1 expression.
 DE    Antigens, CD/*METABOLISM  Cell Adhesion Molecules/*METABOLISM  Cell Line
       Cells, Cultured  Flow Cytometry  Histocompatibility Antigens Class
       I/*METABOLISM  Human  HIV Envelope Protein gp41/*PHARMACOLOGY  *HIV-1
       Interferon-alpha/PHARMACOLOGY  Interferon-beta/PHARMACOLOGY
       Interferon-gamma, Recombinant/PHARMACOLOGY  Major Histocompatibility
       Complex/*DRUG EFFECTS  Monocytes/DRUG EFFECTS/METABOLISM  Receptors,
       Virus/*METABOLISM  Support, Non-U.S. Gov't  T4 Lymphocytes/DRUG
       EFFECTS/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).